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CABRI:ICLC |
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Accession_number |
ICLC ATL95002
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Cell_line_name |
3T3
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Brief_description |
Species: mouse, Swiss albino; Tissue: embryo
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Description |
Species: mouse, Swiss albino; Tissue: embryo
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Depositor |
Obtained from ECACC
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Bibliographic_reference |
J Cell Biol 1963;17:299-313
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Morphology |
fibroblast, grown as monolayer
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Culture_conditions |
continuous culture; DMEM + 10% FBS + 2mM L-Glutamine; Split confluent cultures 1:5-1:10 using trypsin/EDTA; seed at 1-3 x 104 cells/cm2; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
Contact inhibited; transfection studies. The cell line is a valuable in vitro host system for oncogenic transformation studies. It is readily transformed by SV40 and polyoma viruses
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as mouse with G6PD, MD, NP
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Virology 1965;27:179-185 [PMID: 4284655
]
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